Rat Choline Acetyltransferase ELISA Kit is a sandwich (quantitative) Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Choline Acetyltransferase in tissue homogenates, cell lysates or other biological fluids.

Assay-Typ

Sandwich (quantitative)

Assay-Prinzip

Rat Choline Acetyltransferase ELISA Kit employs the Sandwich Enzyme Immunoassay (ELISA) technique for the quantitative measurement of rat Choline Acetyltransferase in rat. An antibody specific for Choline Acetyltransferase has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Choline Acetyltransferase present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Choline Acetyltransferase Antibody, which binds the captured Choline Acetyltransferase present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Choline Acetyltransferase captured in each well. The concentration of Choline Acetyltransferase can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Plattform

Pre-coated Microplate (12 x 8 well strips)

Nachweismethode

Colourmetric

Instrument

Colorimetric Microplate Reader

Probentyp

Tissue homogenates, cell lysates or other biological fluids.

Sensitivität

0.054 ng/ml

Detektionsbereich

0.156-10 ng/ml

Testdauer

1 - 4.5 Hours

Reaktivität

Rat

Recovery

Sample Type	n	Range	Average
Serum	5	80% - 102%	91%
EDTA Plasma	5	81% - 100%	90%
Heparin Plasma	5	80% - 89%	84%

Linearity

Sample Type	1:2	1:4	1:8	1:16
Serum (n=5)	87-91%	87-107%	74-101%	92-97%
EDTA Plasma (n=5)	90-105%	84-101%	90-101%	79-108%
Heparin Plasma (n=5)	84-95%	92-105%	82-105%	89-91%

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Allgemeine Hinweise

Information online is representative. Always refer to the Product Manual inside the kit.

Lagerung

Store at +4°C. Do not use past expiration date!

Synonyms

Acetyl CoA choline O acetyltransferase, Acetyl CoA:choline O acetyltransferase, ChAT, CHOACTase, Choline acetylase, Choline O acetyltransferase, Choline O-acetyltransferase, CLAT_HUMAN, CMS1A, CMS1A2, EC 2.3.1.6, OTTHUMP00000019583, OTTHUMP00000019584

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.