Anti-Raptor Antibody [ARC1375] (A309112)

Name: Anti-Raptor Antibody [ARC1375]
Brand: Antibodies.com
SKU: A309112
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1375] antibody to Raptor for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Raptor Antibody [ARC1375]

Description

Rabbit monoclonal [ARC1375] antibody to Raptor.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1236-1335 of human Raptor (Q8N122).

Sequence

VRIFDPRMPESVNVLQIVKGLTALDIHPQADLIACGSVNQFTAIYNSSGELINNIKYYDGFMGQRVGAISCLAFHPHWPHLAVGSNDYYISVYSVEKRVR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1375

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

150 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

KIAA1303, p150 target of rapamycin, Regulatory-associated protein of mTOR, RPTOR, TOR)-scaffold protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Raptor Antibody [ARC1375] (A309112)

Western blot analysis of extracts of various cell lines, using Anti-Raptor Antibody [ARC1375] (A309112) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Immunohistochemistry - Anti-Raptor Antibody [ARC1375] (A309112)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Raptor Antibody [ARC1375] (A309112) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Raptor Antibody [ARC1375] (A309112)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Raptor Antibody [ARC1375] (A309112) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Raptor Antibody [ARC1375] (A309112)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Raptor Antibody [ARC1375] (A309112) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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