RanGAP1 expression in NIH3T3 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-RanGAP1 Antibody (A83685) at 0.2µg/ml and detected by chemiluminescence.
RanGAP1 expression in Human Liver analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-RanGAP1 Antibody (A83685) at 2.5µg/ml and revealed with alkaline phosphatase (AP).
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