Anti-Raf1 (phospho Ser621) Antikörper (A10884)

Name: Anti-Raf1 (phospho Ser621) Antibody
Brand: Antibodies.com
SKU: A10884
Price: 435.0 USD
Availability: InStock

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$435

Polyklonal Kaninchen Antikörper gegen Raf1 (phospho Ser621) für WB und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

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Produktdetails

Produktname

Anti-Raf1 (phospho Ser621) Antibody

Beschreibung

Rabbit polyclonal antibody to Raf1 (phospho Ser621).

Anwendungen

WB, IP

Verdünnungen

WB: 1:500-1:2,000, IP: 1:50-1:100

Reaktivität

Human

Immunogen

A synthetic phosphorylated peptide around S621 of human Phospho-Raf1-S621 (NP_002871.1).

Sequenz

SASEP

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

73 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

c Raf, C-raf, C-Raf proto-oncogene, serine/threonine kinase, CMD1NN, cRaf, Craf 1 transforming gene, Craf1 transforming gene, EC 2.7.11.1, kinase Raf1, Murine sarcoma 3611 oncogene 1, NS5, Oncogene MIL, Oncogene RAF1, OTTHUMP00000160218, OTTHUMP00000207813, OTTHUMP00000209389, Protein kinase raf 1, Proto-oncogene c-RAF, RAF, Raf 1, Raf 1 proto oncogene serine/threonine kinase, Raf proto oncogene serine/threonine protein kinase, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1_HUMAN, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV, v raf 1 murine leukemia viral oncogene homolog 1, v-raf murine sarcoma viral oncogene homolog 1, v-raf-1 murine leukemia viral oncogene-like protein 1, vraf1 murine leukemia viral oncogene homolog 1

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (2)

Bild Vergrößern

Figure 1: Western Blot - Anti-Raf1 (phospho Ser621) Antibody (A10884)

Western blot analysis of extracts of HeLa and 293T cells, using Anti-Raf1 (phospho Ser621) Antibody (A10884) at 1:1,000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. 293T cells were treated by PMA/TPA (200nM) for 30 minutes or treated by EGF (25ug/mL) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.

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Figure 2: Western Blot - Anti-Raf1 (phospho Ser621) Antibody (A10884)

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-Raf1 (phospho Ser621) Antibody (A10884). This Western blot was performed on the immunoprecipitate using Anti-Raf1 (phospho Ser621) Antibody (A10884) at a dilution of 1:1000. HeLa cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.