Rabbit polyclonal antibody to RAD52 (phospho Tyr104).
Spezifität
This antibody detects endogenous levels of RAD52 only when phosphorylated at Tyr104.
Anwendungen
WB, IHC, ELISA
Verdünnungen
WB: 1:500-1:1000, ELISA: 1:1000
Reaktivität
Human, Mouse
Immunogen
Synthetic peptide derived from human RAD52 around the phosphorylation site of Tyr104 (amino acids 70-119).
Wirt
Rabbit
Klonalität
Polyclonal
Isotyp
IgG
Konjugat
Unconjugated
Reinigung
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molekulargewicht
46kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
DNA repair protein RAD52, DNA repair protein RAD52 homolog, RAD 52, RAD52 homolog, RAD52 homolog (S. cerevisiae), RAD52_HUMAN, Recombination protein RAD52, Rhabdomyosarcoma antigen MU RMS 40.23
Immunohistochemical analysis of paraffin-embedded human testis using Anti-RAD52 (phospho Tyr104) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Figure 2: Western Blot - Anti-RAD52 (phospho Tyr104) Antibody (A93821)
Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30' using Anti-RAD52 (phospho Tyr104) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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