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Anti-Rad51 Antibody (A351)

Western blotting of endogenous Rad51 proteins in crude extracts of varius animal cells. 15% SDS-PAGE was used. Blotting was done at 15 v overnight with wet system. Anti-Rad51 Antibody was used at 1:1,000 dilution. Lane 1: MCF7 (human) cells (40µg). Lane 2: NIH3T3 (mouse) cells (40µg). Lane 3: CHO (hamster) cells (40µg). Lane 4: Xenopus laevis (frog) egg (40µg). Immunoprecipitation of Rad51 protein from crude extract of HeLa cells using Anti-Rad51 Antibody. Anti-Rad51 Antibody (20µg) was incubated with 20µg of HeLa cell extract, and precipitated with 20µg of protein A-beads. The sample was dissociated from the precipitate by heating in SDS-sample buffer and analyzed by western blotting with Anti-Rad51 Antibody (70-005) at 1:1,000 dilution. Rabbit Anti-Chicken IgG Antibody was used as a secondary at 1:10,000 dilution. Detection of Rad51 foci formation after X-ray irradiation in human fibroblast cell line, GM0637. Cells were irradiated by X-rays at 2 Gy, grown for 1 hr, fixed with 4% paraformaldehyde in 1x PBS for 10 min, washed 3 times with PBS for 3 min, permealized by treatment with 0.5% Triton for 5 min, washed 3 times with PBS for 3 min, incubated with Anti-Rad51 Antibody for 30 min at 37°C, washed 3 times with PBS for 3 min, incubated with secondary antibody for 30 min at 37°C, washed 3 times with PBS for 3 min, stained with Hoechst for 1 min and mounted. Anti-Rad51 Antibody was used at 1:10,000 dilution (left panels) and 1:1,000 dilution (right panels). Anti-Rabbit IgG Antibody (Alexa 488) was used as a secondary at 1:10,000 dilution. Immunohistological staining of Rad51 protein in mouse testis using Anti-Rad51 Antibody. A section of formalin fixed and paraffin embedded mouse testis was treated with the Anti-Rad51 Antibody at 1:100 dilution after deparaffization and antigen retrieval. Anti-Rabbit IgG Antibody (Alexa Fluor 647) was used at 1:1,000 dilution. DNA was stained with DAPI and the merged image was shown (Merged). The plain black and white microsopic picture of the same region was shown on the right. Western blot of endogenous Rad52 protein in rat cell lysate. Lane 1: Human MCF7 cell lysate (80µg). Lane 2: Rat PC12 cell lysate (80µg). Proteins in the lysates were separated on SDS-PAGE (12.5 % gel), and blotted to PVDF membrane at 15 V overnight. The membrane was blocked with 3% skim milk. Anti-Rad51 Antibody was used at 1:1,000 dilution and Goat Anti-Rabbit IgG Antibody was used as the secondary at 1:10,000. dilution.
$555

Rabbit polyclonal antibody to Rad51 for WB, IP, ChIP, IF, IHC, DB and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-Rad51 Antibody
Description
Rabbit polyclonal antibody to Rad51.
Applications
WB, IP, ChIP, IF, IHC, DB, ELISA
Dilutions
WB: 1:1,000-1:10,000, IP: 1:1,00-1:1,000, IF: 1:1,000-1:10,000, IHC: 1:1,000-1:5,000, DB: 1:1,000-1:5,000, ELISA: 1:2,000-1:5,000
Reactivity
Human, Mouse, Rat, Chicken, Xenopus
Immunogen
Recombinant full length human Rad51 protein.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DNA repair protein RAD51 homolog 1, hRAD51, HsRAD51, RAD51 homolog A, RAD51A, RECA
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

Western blotting of endogenous Rad51 proteins in crude extracts of varius animal cells. 15% SDS-PAGE was used. Blotting was done at 15 v overnight with wet system. Anti-Rad51 Antibody was used at 1:1,000 dilution. Lane 1: MCF7 (human) cells (40µg). Lane 2: NIH3T3 (mouse) cells (40µg). Lane 3: CHO (hamster) cells (40µg). Lane 4: Xenopus laevis (frog) egg (40µg).Enlarge Image

SDS-PAGE - Anti-Rad51 Antibody (A351)

Anti-Rad51 Antibody
Immunoprecipitation of Rad51 protein from crude extract of HeLa cells using Anti-Rad51 Antibody. Anti-Rad51 Antibody (20µg) was incubated with 20µg of HeLa cell extract, and precipitated with 20µg of protein A-beads. The sample was dissociated from the precipitate by heating in SDS-sample buffer and analyzed by western blotting with Anti-Rad51 Antibody (70-005) at 1:1,000 dilution. Rabbit Anti-Chicken IgG Antibody was used as a secondary at 1:10,000 dilution.Enlarge Image

Immunoprecipitation - Anti-Rad51 Antibody (A351)

Anti-Rad51 Antibody
Detection of Rad51 foci formation after X-ray irradiation in human fibroblast cell line, GM0637. Cells were irradiated by X-rays at 2 Gy, grown for 1 hr, fixed with 4% paraformaldehyde in 1x PBS for 10 min, washed 3 times with PBS for 3 min, permealized by treatment with 0.5% Triton for 5 min, washed 3 times with PBS for 3 min, incubated with Anti-Rad51 Antibody for 30 min at 37°C, washed 3 times with PBS for 3 min, incubated with secondary antibody for 30 min at 37°C, washed 3 times with PBS for 3 min, stained with Hoechst for 1 min and mounted. Anti-Rad51 Antibody was used at 1:10,000 dilution (left panels) and 1:1,000 dilution (right panels). Anti-Rabbit IgG Antibody (Alexa 488) was used as a secondary at 1:10,000 dilution.Enlarge Image

Anti-Rad51 Antibody (A351)

Anti-Rad51 Antibody
Immunohistological staining of Rad51 protein in mouse testis using Anti-Rad51 Antibody. A section of formalin fixed and paraffin embedded mouse testis was treated with the Anti-Rad51 Antibody at 1:100 dilution after deparaffization and antigen retrieval. Anti-Rabbit IgG Antibody (Alexa Fluor 647) was used at 1:1,000 dilution. DNA was stained with DAPI and the merged image was shown (Merged). The plain black and white microsopic picture of the same region was shown on the right.Enlarge Image

Anti-Rad51 Antibody (A351)

Anti-Rad51 Antibody
Publishing research using Anti-Rad51 Antibody (A351)? Please let us know so that we can list the citation on this page.

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