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Anti-Rad51 Antikörper (A350)

SDS-PAGE of Anti-Rad51 Antibody. Western blot analysis of Rad51 in HeLa cell extract (1:2,000 dilution). Detection of Rad51 protein foci formation with Anti-Rad51 Antibody in X-ray irradiated human fibroblast cells, GM0637. 1) The cells were irradiated with X-ray at 2 Gy, grown for 1 hr, fixed with 4% paraformaldehyde for 10 min and washed with PBS three times. 2) Permeabilized by treatment with 0.5% Triton for 5 min and washed with PBS three times. 3) Incubated with Anti-Rad51 Antibody at 1:100 dilution or 1:1,000 dilution for 30 min at 37°C and washed with PBS three times. 4) Anti-Chicken IgY Antibody (Alexa488) was used as a secondary at 1:5,000 dilution. Immunoprecipitation of Rad51 from crude extract of HeLa cells Rad51 was immunoprecipitated from HeLa cell extract, 20 µg, with anti-Rad51 IgY conjugated to Sepharose 4B and the eluate was immunoblotted with rabbit anti-Rad51 antibody (BioAcademia 70-012)
$510

Polyklonal Huhn Antikörper gegen Rad51 für WB, IP, ChIP und IF.

100% Qualitätsgarantie
Preis-Match-Garantie

Versandkosten
$40
Vorlaufzeit
Lieferung in 4-6 Werktagen

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Produktname
Anti-Rad51 Antibody
Beschreibung
Chicken polyclonal antibody to Rad51.
Anwendungen
WB, IP, ChIP, IF
Verdünnungen
WB: 1:1,000-1:5,000, IP: 1:100-1:1,000, IF: 1:100-1:1,000
Reaktivität
Human, Mouse, Rat, Hamster, Xenopus
Immunogen
Recombinant full length human Rad51 protein.
Wirt
Chicken
Klonalität
Polyclonal
Isotyp
IgY
Konjugat

Unconjugated

Reinigung
Affinity purification.
Konzentration
1 mg/ml
Reinheit
> 90% (by SDS-PAGE)
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
BRCA1/BRCA2 containing complex, subunit 5, BRCC 5, BRCC5, DNA repair protein RAD51 homolog 1, DNA repair protein rhp51, FANCR, hRAD51, HsRAD51, HsT16930, MRMV2, Rad 51, RAD51 homolog, RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51 recombinase, RAD51, S. cerevisiae, homolog of, RAD51A, RAD51_HUMAN, RECA, RecA like protein, RecA, E. coli, homolog of, Recombination protein A
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
SDS-PAGE of Anti-Rad51 Antibody.Bild Vergrößern
Figure 1: SDS-PAGE - Anti-Rad51 Antibody (70-005)
Anti-Rad51 Antibody
Western blot analysis of Rad51 in HeLa cell extract (1:2,000 dilution).Bild Vergrößern
Figure 2: Anti-Rad51 Antibody (70-005)
Anti-Rad51 Antibody
Detection of Rad51 protein foci formation with Anti-Rad51 Antibody in X-ray irradiated human fibroblast cells, GM0637. 1) The cells were irradiated with X-ray at 2 Gy, grown for 1 hr, fixed with 4% paraformaldehyde for 10 min and washed with PBS three times. 2) Permeabilized by treatment with 0.5% Triton for 5 min and washed with PBS three times. 3) Incubated with Anti-Rad51 Antibody at 1:100 dilution or 1:1,000 dilution for 30 min at 37°C and washed with PBS three times. 4) Anti-Chicken IgY Antibody (Alexa488) was used as a secondary at 1:5,000 dilution.Bild Vergrößern
Figure 3: Anti-Rad51 Antibody (70-005)
Anti-Rad51 Antibody
Immunoprecipitation of Rad51 from crude extract of HeLa cells Rad51 was immunoprecipitated from HeLa cell extract, 20 µg, with anti-Rad51 IgY conjugated to Sepharose 4B and the eluate was immunoblotted with rabbit anti-Rad51 antibody (BioAcademia 70-012)Bild Vergrößern
Figure 4: Immunoprecipitation - Anti-Rad51 Antibody (70-005)
Anti-Rad51 Antibody

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