Figure 1: Western Blot - Anti-Rad50 Antibody (A11606)
Western blot analysis of extracts of various cell lines, using Anti-Rad50 Antibody (A11606) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Figure 2: Western Blot - Anti-Rad50 Antibody (A11606)
Western blot analysis of extracts of various cell lines, using Anti-Rad50 Antibody (A11606) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Rad50 Antibody (A11606) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 4: Western Blot - Anti-Rad50 Antibody (A11606)
Immunoprecipitation analysis of 150µg extracts of MCF7 cells using 3µg of Anti-Rad50 Antibody (A11606). This Western blot was performed on the immunoprecipitate using Anti-Rad50 Antibody (A11606) at a dilution of 1:500.
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