ELISA of IgGs from different species shows Anti-Rabbit IgG Antibody [RMG03] (Biotin) reacts to rabbit IgG. There is no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50ng/well of different IgG. 500ng/ml, 200ng/ml, or 50ng/ml of Anti-Rabbit IgG Antibody [RMG03] (Biotin) was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of Anti-Rabbit IgG Antibody [RMG03] (Biotin) was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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