Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
GS2, GTP-binding protein Ram, HsT18676, MGC117246, Mutant Ras related protein Rab-27A, Rab-27, RAB-27A, RAB27, RAB27A member RAS oncogene family, RAM, Ras-related protein Rab-27A, Ras-related protein Rab27A, RB27A_HUMAN
Figure 1: Western Blot - Anti-RAB27A Antibody (A13754)
Western blot analysis of extracts from wild type(WT) and RAB27A knockdown (KD) U-87MG(KD) cells, using Anti-RAB27A Antibody (A13754) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of HepG2 cells using Anti-RAB27A Antibody (A13754) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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