Progesterone Receptor (phospho Ser400) Cell Based ELISA Kit
Assay-Typ
Cell-based (qualitative)
Assay-Prinzip
This Progesterone Receptor (phospho Ser400) Cell Based ELISA Kit allows for the detection of Progesterone Receptor (phospho Ser400) and the effects that certain stimulation conditions have on Progesterone Receptor (phospho Ser400) expression in different cell lines. Qualitative determination of Progesterone Receptor (phospho Ser400) concentration is achieved by an indirect ELISA format. In essence, the Progesterone Receptor (phospho Ser400) is captured by Anti-Progesterone Receptor (phospho Ser400) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Progesterone Receptor (phospho Ser400) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-Progesterone Receptor Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Adherent cells and suspension cells.
Detektionsbereich
> 5000 Cells
Testdauer
4 hours 30 minutes
Reaktivität
Human
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at + 4°C. Stable for 6 months.
Synonyms
NR3C3, Nuclear receptor subfamily 3 group C member 3, PGR, PR, PRA, PRB, PRGR_HUMAN, Progestin receptor form A, Progestin receptor form B
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Figure 4: Western Blot - Progesterone Receptor (phospho Ser400) Cell Based ELISA Kit (CBP1208)
The mouse monoclonal antibody to GAPDH is used as a positive control inProgesterone Receptor (phospho Ser400) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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