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Identification of endogenous Pura protein in whole cell extracts of HeLa cells and MCF-7 cells. Arrow indicates the position of PURA bands The anti-Pura antibody was used at 1/1,000 dilution. 12.5% SDS-PAGE was used. Blotting was done in wet system at 15 v overnight. CanGetSignal (Toyobo, Osaka) was used as a signal enhancer
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