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8 ergebnisse für "Primärantikörper" und "Influenza-B-Virus"

8 Products
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B/Florida/4/2006 2. B/Lee/1940 3. B/Malasia/2506/2004 4. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left.
(2)
Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 10B8). Samples were taken at 24 hours post-infection. Anti-Influenza B Virus HA antibody (clone 10B8)) efficiently detected HA in the B/Malasia/2506/2004 virus (Victorial group) but not in B/Florida/4/2006 virus (Yamagata group) infected MDCK cells. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody. Images on the left are mock-infected MDCK cells as negative control.
(2)
Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus NP antibody (clone 8C8). Samples were taken at 24 hours post-infection. Anti-Influenza B Virus NP antibody (clone 8C8) efficiently detected the viruses in the infected MDCK cells. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody. Images on the left are mock-infected MDCK cells as negative control. The cells infected with an Influenza B virus vaccine strain, Malaysia/2506/2004 as a representative of Victoria group is shown in the upper panel and Florida/4/2006 as Yamagata group in the lower panel.

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