Anti-PP2A-alpha Antibody (A15336)

Name: Anti-PP2A-alpha Antibody
Brand: Antibodies.com
SKU: A15336
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to PP2A-alpha for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PP2A-alpha Antibody

Description

Rabbit polyclonal antibody to PP2A-alpha.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:1,000-1:5,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human PP2A Catalytic a (NP_002706.1).

Sequence

MDEKVFTKELDQWIEQLNECKQLSESQVKSLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

36 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

PPP2CA, Replication protein C, RP-C, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-PP2A-alpha Antibody (A15336)

Western blot analysis of various lysates, using Anti-PP2A-alpha Antibody (A15336) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunofluorescence - Anti-PP2A-alpha Antibody (A15336)

Immunofluorescence analysis of HeLa cells using Anti-PP2A-alpha Antibody (A15336) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-PP2A-alpha Antibody (A15336)

Immunofluorescence analysis of NIH/3T3 cells using Anti-PP2A-alpha Antibody (A15336) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-PP2A-alpha Antibody (A15336)

Immunoprecipitation analysis of 200µg extracts of 293T cells using 3µg of Anti-PP2A-alpha Antibody (A15336). This Western blot was performed on the immunoprecipitate using Anti-PP2A-alpha Antibody (A15336) at a dilution of 1:1000.