Anti-POLD3 Antikörper (A10090)

Name: Anti-POLD3 Antibody
Brand: Antibodies.com
SKU: A10090
Price: 335.0 USD
Availability: InStock

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$335

Polyklonal Kaninchen Antikörper gegen POLD3 für WB, IHC und ICC/IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Produktdetails

Produktname

Anti-POLD3 Antibody

Beschreibung

Rabbit polyclonal antibody to POLD3.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:500-1:2,000, ICC/IF: 1:50-1:100, IHC: 1:100-1:500

Reaktivität

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 197-466 of human POLD3 (NP_006582.1).

Sequenz

ASKAAAKTQETNKETKTEAKEVTNASAAGNKAPGKGNMMSNFFGKAAMNKFKVNLDSEQAVKEEKIVEQPTVSVTEPKLATPAGLKKSSKKAEPVKVLQKEKKRGKRVALSDDETKETENMRKKRRRIKLPESDSSEDEVFPDSPGAYEAESPSPPPPPSPPLEPVPKTEPEPPSVKSSSGENKRKRKRVLKSKTYLDGEGCIVTEKVYESESCTDSEEELNMKTSSVHRPPAMTVKKEPREERKGPKKGTAALGKANRQVSITGFFQRK

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

51 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

DNA polymerase delta subunit 3, DNA polymerase delta subunit p66, DNA polymerase delta, subunit 3, DNA polymerase subunit delta p66, DPOD3_HUMAN, KIAA0039, P66, P68, Pol delta C subunit (p66), Polymerase (DNA directed), delta 3, Polymerase (DNA-directed), delta 3, accessory subunit, PPP1R128, Protein phosphatase 1, regulatory subunit 128

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

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Figure 1: Western Blot - Anti-POLD3 Antibody (A10090)

Western blot analysis of extracts of various cell lines, using Anti-POLD3 Antibody (A10090) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-POLD3 Antibody (A10090)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-POLD3 Antibody (A10090) at a dilution of 1:300 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-POLD3 Antibody (A10090)

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using Anti-POLD3 Antibody (A10090). Green:GFP-RNF168 fusion protein expression for DNA damage marker. DAPI was used to stain the cell nuclei (blue). RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.