Western Blot - Anti-PKC delta (phospho Tyr311) Antibody (A10976)
Western blot analysis of extracts of U937 and Raw264. 7 cells, using Anti-PKC delta (phospho Tyr311) Antibody (A10976) at 1:1,000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. RAW 264. 7 cells were treated by PMA (200 nM) for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.
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