Figure 1: Western Blot - Anti-PIM2 Antibody (A84957)
PIM2 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress MYC tagged PIM2 an lysed in RIPA buffer. Primary incubation was performed for 1 hour on 10µg protein per lane with Anti-PIM2 Antibody (A84957) at 0.1µg/ml (Lane A) or Anti-MYC at 1:1000 dilution (Lane C) and detected with chemiluminescence. Control: Mock-transfected HEK293 cells incubated with Anti-PIM2 Antibody (A84957) at 0.1mg/ml (Lane B).
PIM2 expression in Human Heart analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-PIM2 Antibody (A84957) at 5µg/ml and revealed with alkaline phosphatase (AP).
Alternative Produkte zum Anti-PIM2 Antikörper (A84957)