Anti-PGC1 alpha Antikörper (A326276)

Name: Anti-PGC1 alpha Antibody
Brand: Antibodies.com
SKU: A326276
Price: 448.0 USD
Availability: InStock

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$448

Polyklonal Ziege Antikörper gegen PGC1 alpha für ELISA, WB, IF und Flow Cytometry.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-PGC1 alpha Antibody

Beschreibung

Goat polyclonal antibody to PGC1 alpha.

Anwendungen

ELISA, WB, IF, Flow Cytometry

Verdünnungen

ELISA: 1:128,000, WB: 1.5-3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reaktivität

Human

Immunogen

Synthetic peptide corresponding to the internal region of human PGC1 alpha.

Sequenz

RDSVSPPKSLFSQC

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Molekulargewicht

70+90 kDa

Geschätztes MW

91.0kDa

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

L PGC 1alpha, LEM6, Ligand effect modulator 6, Peroxisome proliferative activated receptor gamma coactivator 1, Peroxisome proliferative activated receptor gamma coactivator 1 alpha, Peroxisome proliferator activated receptor gamma coactivator 1 alpha, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PGC 1 (alpha), PGC 1 alpha, PGC 1v, PGC-1-alpha, PGC1, PGC1(alpha), PGC1A, PGC1v, PPAR gamma coactivator 1, PPAR gamma coactivator 1 alpha, PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6, PPAR gamma coactivator variant form, PPAR-gamma coactivator 1-alpha, PPARGC 1 alpha, PPARGC-1-alpha, PPARGC1, PPARGC1A, PRGC1_HUMAN

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (8)

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Figure 1: Western Blot - Anti-PGC1 alpha Antibody (A326276)

PGC1 alpha expression in A431 (A), HepG2 (B), and HEK293 nuclear (C) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-PGC1 alpha Antibody (A326276) at 3µg/ml (A-B) or 1.5µg/ml (C) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-PGC1 alpha Antibody (A326276)

PGC1 alpha expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PGC1 alpha Antibody (A326276) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-PGC1 alpha Antibody (A326276)

PGC1 alpha expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PGC1 alpha Antibody (A326276) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-PGC1 alpha Antibody (A326276)

PGC1 alpha expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-PGC1 alpha Antibody (A326276) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.