Recombinant mouse monoclonal [rPRF1/8058] antibody to Perforin.
Spezifität
Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.
Anwendungen
WB, IHC-P
Positivkontrolle
HDLM-2 cells. Human spleen.
Verdünnungen
WB: 1-2 µg/ml, IHC: 1-2 µg/ml
Reaktivität
Human
Immunogen
Recombinant human Perforin-1 protein fragment (around Amino Acids 355-555). The exact sequence is proprietary.
Wirt
Mouse
Klonalität
Monoclonal
Klon
rPRF1/8058
Isotyp
IgG1
Leichte Ketten
kappa
Konjugat
Unconjugated
Reinigung
Protein A/G chromatography.
Konzentration
200 µg/ml
Molekulargewicht
75 kDa
Produktform
Liquid
Formulierung
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.