This antibody recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
Anwendungen
Flow Cytometry
Verdünnungen
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests; Intracellular staining.
Reaktivität
Human, Bovine
Kreuzreaktivität
This antibody does not cross react with Mouse.
Immunogen
Purified granules from human YT lymphoma cell line.
Wirt
Mouse
Klonalität
Monoclonal
Klon
dG9
Isotyp
IgG2b
Leichte Ketten
kappa
Konjugat
PE
Excitation: 565nm, Emission: 578nm
Reinigung
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Geschätztes MW
70 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Lagerung
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!