This antibody recognises perforin a 60-70 kDa glycoprotein largely expressed in CD8+ cytotoxin T lymphocytes and Natural Killer cells. It aids in defense against virus-infected or neoplastic cells by inducing apoptosis. Perforin achieves this by inserting a portion of its C-terminus region into the lipid membrane of the target in a calcium dependent manner. Subsequent polymerization with other perforin molecules forms a pore in the membrane. These pores allow the free influx and efflux of ions and proteins though the cell membrane. This disruption to homeostasis results in tonic shock which can ultimately lead to apoptosis and DNA degradation. Genetic abnormalities to the perforin gene have been shown to be linked to abnormal immune system functioning, the development of immune diseases as well as lymphomas and leukemias. This clone dG9 has been used in flow cytometry experiments to examine intracellular perforin in tumour specific CD8+ T cells incubated with autologous primary breast cancer cells and Tregs.
Anwendungen
Flow Cytometry
Verdünnungen
FC 1: Neat, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reaktivität
Human, Bovine
Kreuzreaktivität
This antibody does not cross react with Mouse
Immunogen
Purified granules from human YT lymphoma cell line.
Wirt
Mouse
Klonalität
Monoclonal
Klon
dG9
Isotyp
IgG2b
Konjugat
PE
Excitation: 565nm, Emission: 578nm
Reinigung
Protein A affinity chromatography of tissue culture supernatant.
Konzentration
Lot Specific
Reinheit
>95% (by SDS-PAGE).
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline with 0.2% BSA and <0.1% Sodium Azide.
Lagerung
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light.
Allgemeine Hinweise
Mouse anti Human perforin, clone dG9 recognizes perforin a 60-70 kDa glycoprotein largely expressed in CD8+ cytotoxin T lymphocytes and Natural Killer cells. It aids in defense against virus-infected or neoplastic cells by inducing apoptosis. Perforin achieves this by inserting a portion of its C-terminus region into the lipid membrane of the target in a calcium dependent manner. Subsequent polymerization with other perforin molecules forms a pore in the membrane. These pores allow the free influx and efflux of ions and proteins though the cell membrane. This disruption to homeostasis results in tonic shock which can ultimately lead to apoptosis and DNA degradation (Osinska et al. 2014). Genetic abnormalities to the perforin gene have been shown to be linked to abnormal immune system functioning, the development of immune diseases as well as lymphomas and leukemias (Osinska et al. 2014). This clone dG9 has been used in flow cytometry experiments to examine intracellular perforin in tumour specific CD8+ T cells incubated with autologous primary breast cancer cells and Tregs (Su et al. 2017).