Anti-PDPK1 Antikörper (A13582)

Name: Anti-PDPK1 Antibody
Brand: Antibodies.com
SKU: A13582
Price: 470.0 USD
Availability: InStock

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$470

Polyklonal Kaninchen Antikörper gegen PDPK1 für WB und IHC.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Stichworte:

Knockout-validierter Antikörper

Produktdetails

Produktname

Anti-PDPK1 Antibody

Beschreibung

Rabbit polyclonal antibody to PDPK1.

Anwendungen

WB, IHC

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 409-556 of human PDK1/PDPK1 (NP_002604.1).

Sequenz

GSNIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKMGPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

58 - 68 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

3 phosphoinositide dependent protein kinase 1, 3-phosphoinositide-dependent protein kinase 1, hPDK 1, hPDK1, MGC20087, MGC35290, OTTHUMP00000159109, OTTHUMP00000159110, OTTHUMP00000174525, PDK1, PDPK1_HUMAN, PDPK2, PDPK2P, PkB kinase, PkB kinase like gene 1, PkB like 1, PRO0461, Protein kinase

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Western Blot - Anti-PDPK1 Antibody (A13582)

Western blot analysis of extracts of various cell lines, using Anti-PDPK1 Antibody (A13582) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-PDPK1 Antibody (A13582)

Western blot analysis of extracts from normal (control) and PDK1/PDPK1 knockout (KO) HCT116 cells, using Anti-PDPK1 Antibody (A13582) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-PDPK1 Antibody (A13582)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-PDPK1 Antibody (A13582) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.