Anti-PDGF BB Antibody [AB13-3G6] (Biotin) (A279626)

Anti-PDGF BB Antibody [AB13-3G6] (Biotin)

Mouse monoclonal [AB13-3G6] antibody to PDGF BB (Biotin).

This antibody recognises the homodimer of platelet-derived growth factor subunit B (PDGF-B) also known as PDGF-2, PDGF beta chain and PDGF beta polypeptide. PDGF-BB is a member of the PDGF growth factor family which is made up of 4 isoforms (A, B, C and D). PDGF-BB is a homodimer of the B isoform which is able to bind all three of the PDGF receptors to trigger various signaling pathways including Ras-MAPK, PI3K, PLC?, and JAK. Activation of these pathways lead to transcription of PDGF target genes playing roles in embryogenesis, and in adults under physiological conditions, roles in tissue repair, wound healing and maturation of blood vessels and white adipocytes. The biotinylated Mouse anti Human PDGF-BB antibody, clone AB13-3G6 (MCA6265B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human PDGF-BB antibody, clone AB06-1E10 (MCA6264GA) as the capture antibody.

ELISA: 1.0 ug/lm

Human

E. coli derived recombinant human full length PDGF-BB (Ser82-Thr190).

Mouse

Monoclonal

AB13-3G6

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human PDGF-BB antibody, clone AB13-3G6, recognizes the homodimer of platelet-derived growth factor subunit B (PDGF-B) also known as PDGF-2, PDGF beta chain and PDGF beta polypeptide. PDGF-BB is a member of the PDGF growth factor family which is made up of 4 isoforms (A, B, C and D). PDGF-BB is a homodimer of the B isoform which is able to bind all three of the PDGF receptors to trigger various signaling pathways including Ras-MAPK, PI3K, PLC?, and JAK (Evrova & Buschmann 2017). Activation of these pathways lead to transcription of PDGF target genes playing roles in embryogenesis, and in adults under physiological conditions, roles in tissue repair, wound healing and maturation of blood vessels and white adipocytes (Saplacan et al. 2017). The biotinylated Mouse anti Human PDGF-BB antibody, clone AB13-3G6 (MCA6265B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human PDGF-BB antibody, clone AB06-1E10 (MCA6264GA) as the capture antibody.

This product is for research use only. It is not intended for diagnostic or therapeutic use.