Anti-PARP1 Antikörper (A308167)

Name: Anti-PARP1 Antibody
Brand: Antibodies.com
SKU: A308167
Price: 470.0 USD
Availability: InStock

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$470

Polyklonal Kaninchen Antikörper gegen PARP1 für WB, IHC und ICC/IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Stichworte:

Knockout-validierter Antikörper

Produktdetails

Produktname

Anti-PARP1 Antibody

Beschreibung

Rabbit polyclonal antibody to PARP1.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:2,000-1:6,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human PARP1 (NP_001609.2).

Sequenz

PGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSG

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

114 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

ADP ribosyltransferase, ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase), ADP ribosyltransferase diphtheria toxin like 1, ADP ribosyltransferase NAD(+), ADPRT, ADPRT 1, ADPRT1, ARTD1, msPARP, NAD(+) ADP ribosyltransferase 1, NAD(+) ADP-ribosyltransferase 1, pADPRT 1, pADPRT1, PARP, PARP 1, PARP-1, PARP1_HUMAN, Poly (ADP ribose) polymerase 1, poly (ADP ribose) polymerase family, member 1, Poly [ADP-ribose] polymerase 1, Poly(ADP ribose) polymerase, poly(ADP ribose) synthetase, poly(ADP ribosyl)transferase, Poly[ADP ribose] synthetase 1, Poly[ADP-ribose] synthase 1, PPOL

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (5)

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Figure 1: Western Blot - Anti-PARP1 Antibody (A308167)

Western blot analysis of extracts from wild type(WT) and PARP1 knockout (KO) HeLa cells, using Anti-PARP1 Antibody (A308167) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-PARP1 Antibody (A308167)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PARP1 Antibody (A308167) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-PARP1 Antibody (A308167)

Immunofluorescence analysis of C6 cells using Anti-PARP1 Antibody (A308167) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-PARP1 Antibody (A308167)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PARP1 Antibody (A308167) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).