Anti-PAPP A Antibody [10E1] (A278772)

Anti-PAPP A Antibody [10E1]

Mouse monoclonal [10E1] antibody to PAPP A.

This antibody recognises human pregnancy-associated plasma protein A, or PAPP-A, a metalloprotease specific for insulin-like growth factor binding protein-4 and -5 (IGFBP4 and IGFBP5). PAPP-A was initially identified as a high-molecular mass component in human pregnancy serum. In pregnancy serum PAPP-A exists in a disulfide bound heterotetrameric complex consisting of two ~200 kDa PAPP-A subunits and two ~50-90 kDa subunits of the proform of eosinophil major basic protein (proMBP). The concentration of PAPP-A in serum increases with gestational age, however, in Down’s syndrome pregnancies PAPP-A concentration is markedly reduced in the first trimester. As such, PAPP-A is widely used as an early marker for Down’s syndrome. PAPP-A has also been shown to exist in a homodimeric, 400 kDa form in unstable atherosclerotic plaques. Increased circulating concentrations of this dimeric PAPP-A are associated with acute coronary syndromes. recognises both the heterotetrameric complex (PAPP-A associated with proMBP) and the homodimeric complex of PAPP-A.

WB, ELISA, IP

Human

Purified human PAPP-A/proMBP complex.

Mouse

Monoclonal

10E1

IgG2b

Protein A affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human PAPP-A antibody, clone 10E1 recognizes human pregnancy-associated plasma protein A, or PAPP-A, a metalloprotease specific for insulin-like growth factor binding protein-4 and -5 (IGFBP4 and IGFBP5). PAPP-A was initially identified as a high-molecular mass component in human pregnancy serum. In pregnancy serum PAPP-A exists in a disulfide bound heterotetrameric complex consisting of two ~200 kDa PAPP-A subunits and two ~50-90 kDa subunits of the proform of eosinophil major basic protein (proMBP). The concentration of PAPP-A in serum increases with gestational age, however, in Down’s syndrome pregnancies PAPP-A concentration is markedly reduced in the first trimester. As such, PAPP-A is widely used as an early marker for Down’s syndrome. PAPP-A has also been shown to exist in a homodimeric, 400 kDa form in unstable atherosclerotic plaques. Increased circulating concentrations of this dimeric PAPP-A are associated with acute coronary syndromes. Mouse anti Human PAPP-A antibody, clone 10E1 recognizes both the heterotetrameric complex (PAPP-A associated with proMBP) and the homodimeric complex of PAPP-A.

IGF-dependent IGFBP-4 protease, IGFBP-4ase, Insulin-like growth factor-dependent IGF-binding protein 4 protease, PAPP-A, PAPPA, Pappalysin-1, Pregnancy-associated plasma protein A