Pancreatic Polypeptide expression in Human Pancreas analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in Tris/EDTA buffer, pH 6. Staining was performed with Anti-Pancreatic Polypeptide Antibody (A84102) at 3µg/ml and revealed with horseradish peroxidase (HRP).
Pancreatic Polypeptide expression in Human Pancreas analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Pancreatic Polypeptide Antibody (A84102) at 5µg/ml and revealed with alkaline phosphatase (AP).
Pancreatic Polypeptide expression in Human Intestine analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Pancreatic Polypeptide Antibody (A84102) at 5µg/ml and revealed with alkaline phosphatase (AP).
Pancreatic Polypeptide expression in U2OS cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Pancreatic Polypeptide Antibody (A84102) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
