Mouse monoclonal [BP53-12] antibody to p53 (FITC).
Specificity
This antibody recognizes a defined epitope, amino acids 16-25, on human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells; it is frequently mutated or inactivated in many types of cancer.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 3 µg/ml; Intracellular staining.
Reactivity
Human, Non-Human Primates
Immunogen
Bacterially expressed full length wild-type p53 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
BP53-12
Isotype
IgG2a
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
1 mg/ml
Predicted MW
50 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!