Anti-p53 Antikörper (A309435)

Name: Anti-p53 Antibody
Brand: Antibodies.com
SKU: A309435
Price: 470.0 USD
Availability: InStock

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$470

Polyklonal Kaninchen Antikörper gegen p53 für WB, IHC, ICC/IF, IP und ChIP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Stichworte:

Knockout-validierter Antikörper ER Marker Mitochondrial Marker

Produktdetails

Produktname

Anti-p53 Antibody

Beschreibung

Rabbit polyclonal antibody to p53.

Anwendungen

WB, IHC, ICC/IF, IP, ChIP

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200, IP: 1:1,000-1:5,000, ChIP: 1:1,000-1:5,000

Reaktivität

Human

Immunogen

Recombinant protein of human p53.

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

53 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (7)

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Figure 1: Western Blot - Anti-p53 Antibody (A309435)

Western blot analysis of extracts of 293T cells, using Anti-p53 Antibody (A309435) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-p53 Antibody (A309435)

Western blot analysis of extracts from wild type (WT) and p53 knockout (KO) 293T cells, using Anti-p53 Antibody (A309435) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-p53 Antibody (A309435)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-p53 Antibody (A309435) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-p53 Antibody (A309435)

Confocal immunofluorescence analysis of Hela cells using Anti-p53 Antibody (A309435) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).