Figure 1: Western Blot - Anti-p40-phox Antibody [ARC2553] (A306699)
Western blot analysis of extracts of THP-1 cells, using Anti-p40-phox Antibody [ARC2553] (A306699) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 2: Western Blot - Anti-p40-phox Antibody [ARC2553] (A306699)
Western blot analysis of extracts of Mouse thymus, using Anti-p40-phox Antibody [ARC2553] (A306699) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-p40-phox Antibody [ARC2553] (A306699) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Figure 4: Western Blot - Anti-p40-phox Antibody [ARC2553] (A306699)
Immunoprecipitation analysis of 300µg extracts of THP-1 cells using 3µg of Anti-p40-phox Antibody [ARC2553] (A306699). This Western blot was performed on the immunoprecipitate using Anti-p40-phox Antibody [ARC2553] (A306699) at a dilution of 1:1000.
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