Figure 1: Western Blot - Anti-p38 alpha / MAPK14 Antibody (A89761)
Western blot analysis of extracts of various cell lines, using Anti-p38 alpha / MAPK14 Antibody (A89761) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-p38 alpha / MAPK14 Antibody (A89761) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-p38 alpha / MAPK14 Antibody (A89761) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using Anti-p38 alpha / MAPK14 Antibody (A89761) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Figure 5: Western Blot - Anti-p38 alpha / MAPK14 Antibody (A89761)
Immunoprecipitation analysis of 200µg extracts of U-87MG cells using 3µg of Anti-p38 alpha / MAPK14 Antibody (A89761). This Western blot was performed on the immunoprecipitate using Anti-p38 alpha / MAPK14 Antibody (A89761) at a dilution of 1:1000.
