Anti-p21 Antibody [CIP1/4377R] - BSA and Azide free
Description
Recombinant rabbit monoclonal [CIP1/4377R] antibody to p21.
Specificity
This antibody recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This antibody is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
Applications
IHC-P
Dilutions
IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to amino acids 1-100 of p21 protein.
Host
Rabbit
Clonality
Monoclonal
Clone ID
CIP1/4377R
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
21 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Anti-p21 Antibody [CIP1/4377R] - BSA and Azide free (A251274)
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-p21 Antibody [CIP1/4377R]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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