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Anti-NUP214 Antibody (A12242)

Western Blot - Anti-NUP214 Antibody (A12242) - Antibodies.com Immunofluorescence - Anti-NUP214 Antibody (A12242) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to NUP214 for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-NUP214 Antibody
Description
Rabbit polyclonal antibody to NUP214.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 750-1030 of human NUP214 (NP_005076.3).
Sequence
FLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDQHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYKQTSLWSLSSAVPSQSSIHSFDSDLESLCNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSCKDDEAVVQAPRHAPVVRTPSIQPSLLP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
251 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
214 kDa nucleoporin, CAIN, CAN, KIAA0023, Nuclear pore complex protein Nup214, Nucleoporin Nup214, Protein CAN
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-NUP214 Antibody (A12242) - Antibodies.comEnlarge Image

Western Blot - Anti-NUP214 Antibody (A12242)

Western blot analysis of extracts of various cell lines, using Anti-NUP214 Antibody (A12242) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunofluorescence - Anti-NUP214 Antibody (A12242) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-NUP214 Antibody (A12242)

Immunofluorescence analysis of L929 cells using Anti-NUP214 Antibody (A12242) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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