Anti-Nucleolin Antibody [NCL/7338] - BSA and Azide free
Beschreibung
Mouse monoclonal [NCL/7338] antibody to Nucleolin.
Spezifität
This antibody recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Anwendungen
IHC-P
Verdünnungen
IHC-P: 1-2 µg/ml
Reaktivität
Human
Kreuzreaktivität
This antibody does not cross react with Mouse, Feline, Bovine, Canine, or Horse.
Immunogen
Recombinant full-length human NCL protein.
Wirt
Mouse
Klonalität
Monoclonal
Klon
NCL/7338
Isotyp
IgG2b
Leichte Ketten
kappa
Konjugat
Unconjugated
Reinigung
Protein A/G chromatography.
Konzentration
1 mg/ml
Molekulargewicht
76 kDa
Produktform
Liquid
Formulierung
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue using Anti-Nucleolin Antibody [NCL/7338]. Inset: PBS instead of the primary antibody. Secondary antibody negative control.
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