Anti-Notch1 Antikörper [S253-32] (A304795)

Name: Anti-Notch1 Antibody [S253-32]
Brand: Antibodies.com
SKU: A304795
Price: 500.0 USD
Availability: InStock

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$500

Monoklonal [S253-32] Maus Antikörper gegen Notch1 für WB und ICC/IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Stichworte:

Neuroepithelial Marker

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Anti-Notch1 Antikörper [mN1A]

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Produktdetails

Produktname

Anti-Notch1 Antibody [S253-32]

Beschreibung

Mouse monoclonal [S253-32] antibody to Notch1.

Anwendungen

WB, ICC/IF

Verdünnungen

WB: 1:1,000

Reaktivität

Human, Mouse, Rat

Kreuzreaktivität

This antibody does not cross-react with Notch 2 or Notch 3.

Immunogen

Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1.

Wirt

Mouse

Klonalität

Monoclonal

Klon

S253-32

Isotyp

IgG1

Konjugat

Unconjugated

Reinigung

Protein G purification.

Konzentration

1 mg/ml

Molekulargewicht

>270 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

9930111A19Rik, AOS5, AOVD1, hN1, Lin-12, LIN12, Mis6, Motch A, mT14, Neurogenic locus notch homolog protein 1, Neurogenic locus notch protein homolog, NICD, NOTC1_HUMAN, NOTCH, Notch 1, Notch 1 intracellular domain, Notch gene homolog 1 (Drosophila), Notch homolog 1, translocation-associated (Drosophila), NOTCH, Drosophila, homolog of, 1, TAN1, Translocation associated notch homolog, Translocation associated notch protein TAN 1, Translocation-associated notch protein TAN-1, xotch

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Immunofluorescence - Anti-Notch1 Antibody [S253-32] (A304795)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-Notch1 Antibody [S253-32] (A304795), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Notch1 Antibody (D) Composite.

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Figure 2: Western Blot - Anti-Notch1 Antibody [S253-32] (A304795)

Western blot analysis of rat brain membrane showing detection of ~270 kDa Notch1 protein using Anti-Notch1 Antibody [S253-32] (A304795) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: rat brain membrane (10 µg).. Load: 10 µg. Block: 5% milk. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at room temperature. Color Development: TMB solution for 10 min at room temperature. Predicted/Observed Size: ~270 kDa.

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Figure 3: Immunofluorescence - Anti-Notch1 Antibody [S253-32] (A304795)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Notch1 Antibody [S253-32] (A304795), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cell Membrane, Nucleus. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Notch1 Antibody. (D) Composite.