Figure 1: Western Blot - Anti-NNMT Antibody (A9963)
Western blot analysis of extracts of A-549 cells, using Anti-NNMT Antibody (A9963) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of HepG2 cells using Anti-NNMT Antibody (A9963) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-NNMT Antibody (A9963) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-NNMT Antibody (A9963) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-NNMT Antibody (A9963) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).