Western Blot - Anti-NF-kB p65 (phospho Thr434) Antibody (A91503)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-NF-kB p65 (phospho Thr434) Antibody (A91503) at 1:1,000 dilution or RelA antibody (A16271). NIH/3T3 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 5min.
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