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Anti-NF-kappaB p65 (phospho Thr254) Antibody (A93733)

Western Blot - Anti-NF-kappaB p65 (phospho Thr254) Antibody (A7172) - Antibodies.com Immunohistochemistry - Anti-NF-kappaB p65 (phospho Thr254) Antibody (A7172) - Antibodies.com Western Blot - Anti-NF-kappaB p65 (phospho Thr254) Antibody (A7172) - Antibodies.com
$190
Product Datasheet

Rabbit polyclonal antibody to NF-kappaB p65 (phospho Thr254) for WB, IHC, IP and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-NF-kappaB p65 (phospho Thr254) Antibody
Description
Rabbit polyclonal antibody to NF-kappaB p65 (phospho Thr254).
Specificity
This antibody detects endogenous levels of NF-kappaB p65 only when phosphorylated at Thr254.
Applications
WB, IHC, IP, ELISA
Dilutions
WB: 1:500-1:1000, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human NF-kappaB p65 around the phosphorylation site of Thr254 (amino acids 221-270).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
60kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
NFKB3, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, Transcription factor p65
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-NF-kappaB p65 (phospho Thr254) Antibody (A7172) - Antibodies.comEnlarge Image

Western Blot - Anti-NF-kappaB p65 (phospho Thr254) Antibody (A93733)

Western blot analysis of lysates from 293 cells treated with TNF-alpha using Anti-NF-kappaB p65 (phospho Thr254) Antibody. The left hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemistry - Anti-NF-kappaB p65 (phospho Thr254) Antibody (A7172) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-NF-kappaB p65 (phospho Thr254) Antibody (A93733)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-NF-kappaB p65 (phospho Thr254) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-NF-kappaB p65 (phospho Thr254) Antibody (A7172) - Antibodies.comEnlarge Image

Western Blot - Anti-NF-kappaB p65 (phospho Thr254) Antibody (A93733)

Western blot analysis of various cells using Anti-NF-kappaB p65 (phospho Thr254) Antibody.
Publishing research using Anti-NF-kappaB p65 (phospho Thr254) Antibody (A93733)? Please let us know so that we can list the citation on this page.

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