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Anti-Neutrophil Elastase Antikörper [ARC1364] (A309860)

Western Blot - Anti-Neutrophil Elastase Antibody [ARC1364] (A309860) - Antibodies.com Immunohistochemistry - Anti-Neutrophil Elastase Antibody [ARC1364] (A309860) - Antibodies.com Immunofluorescence - Anti-Neutrophil Elastase Antibody [ARC1364] (A309860) - Antibodies.com
$470
Product Datasheet

Monoklonal [ARC1364] Kaninchen Antikörper gegen Neutrophil Elastase für WB, IHC, ICC/IF und IP.

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Preis-Match-Garantie

Versandkosten
$40
Vorlaufzeit
Lieferung in 5-8 Werktagen

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Produktname
Anti-Neutrophil Elastase Antibody [ARC1364]
Beschreibung
Rabbit monoclonal [ARC1364] antibody to Neutrophil Elastase.
Anwendungen
WB, IHC, ICC/IF, IP
Verdünnungen
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200
Reaktivität
Human, Mouse
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 167-267 of human Neutrophil Elastase (ELANE) (P08246).
Wirt
Rabbit
Klonalität
Monoclonal
Klon
ARC1364
Isotyp
IgG
Konjugat

Unconjugated

Reinigung
Affinity purification.
Molekulargewicht
30 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
Bone marrow serine protease, ELA2, ELANE, Elastase 2, Elastase 2 neutrophil, Elastase neutrophil expressed, Elastase-2, ELNE_HUMAN, GE, Granulocyte derived elastase, HLE, HNE, Human leukocyte elastase, Leukocyte elastase, Medullasin, NE, PMN E, PMN elastase, Polymorphonuclear elastase, SCN1
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western Blot - Anti-Neutrophil Elastase Antibody [ARC1364] (A309860) - Antibodies.comBild Vergrößern
Figure 1: Western Blot - Anti-Neutrophil Elastase Antibody [ARC1364] (A309860)
Western blot analysis of extracts of various cell lines, using Anti-Neutrophil Elastase Antibody [ARC1364] (A309860) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry - Anti-Neutrophil Elastase Antibody [ARC1364] (A309860) - Antibodies.comBild Vergrößern
Figure 2: Immunohistochemistry - Anti-Neutrophil Elastase Antibody [ARC1364] (A309860)
Immunohistochemistry analysis of paraffin-embedded mouse bone marrow using Anti-Neutrophil Elastase Antibody [ARC1364] (A309860) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Neutrophil Elastase Antibody [ARC1364] (A309860) - Antibodies.comBild Vergrößern
Figure 3: Immunofluorescence - Anti-Neutrophil Elastase Antibody [ARC1364] (A309860)
Immunofluorescence analysis of human spleen using Anti-Neutrophil Elastase Antibody [ARC1364] (A309860) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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