Figure 1: Western Blot - Anti-Neuropeptide Y Antibody (A14393)
Western blot analysis of extracts of SH-SY5Y cells, using Anti-Neuropeptide Y Antibody (A14393) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Figure 2: Immunohistochemistry - Anti-Neuropeptide Y Antibody (A14393)
Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-Neuropeptide Y Antibody (A14393) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Figure 3: Immunofluorescence - Anti-Neuropeptide Y Antibody (A14393)
Immunofluorescence analysis of SH-SY5Y cells using Anti-Neuropeptide Y Antibody (A14393) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Alternative Produkte zum Anti-Neuropeptide Y Antikörper (A14393)
