Anti-NeuN Antibody [NeuN/6694R] - BSA and Azide free
Beschreibung
Recombinant rabbit monoclonal [NeuN/6694R] antibody to NeuN.
Spezifität
NeuN antibody specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
Anwendungen
IHC-P
Verdünnungen
IHC-P: 1-2 µg/ml
Reaktivität
Human
Immunogen
Synthetic peptide, within amino acids 30-60, of human NeuN protein. The exact sequence is proprietary.
Wirt
Rabbit
Klonalität
Monoclonal
Klon
NeuN/6694R
Isotyp
IgG
Leichte Ketten
kappa
Konjugat
Unconjugated
Reinigung
Protein A/G chromatography.
Konzentration
1 mg/ml
Molekulargewicht
38 kDa
Produktform
Liquid
Formulierung
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue using Anti-NeuN Antibody [NeuN/6694R]. Inset: PBS instead of the primary antibody. Secondary antibody negative control.