Figure 1: Western Blot - Anti-Nanog Antibody (A84315)
Nanog expression in HeLa nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Nanog Antibody (A84315) at 0.05µg/ml and detected by chemiluminescence.
Nanog expression in Human Testis analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Nanog Antibody (A84315) at 2µg/ml and revealed with alkaline phosphatase (AP).
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