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Anti-NAMPT Aptamer (Biotin) (A320917)

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Name
Anti-NAMPT Aptamer (Biotin)
Description
Synthetic DNA aptamer to NAMPT (Biotin).
Specificity
No significant signal was observed from RBP4, Vaspin, adiponectin, human serum albumin, human IgG, and fibrinogen.
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Binding Buffer: 25mM Tris-HCl Buffer, pH 7.6, with 100mM Sodium Chloride, 2mM Magnesium Chloride, 5mM Potassium Chloride, 1mM Calcium Chloride, and 0.02% Tween 20. Binding Temperature: 25°C.
Sequence
5'-dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdGpdGpdGpdCpdApdGpdGpdApdCpdApdGpdGpdTpdGpdTpdCpdGpdGpdCpdTpdTpdGpdApdTpdApdGpdGpdCpdTpdGpdGpdGpdGpdTpdGpdTpdGpdTpdGpdTpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp-3'
Aptamer Length
76 nt
Aptamer Molecular Weight (Da)
23,679.45 Da
Aptamer Affinity
Affinity (Kd) = 72.52 nM
Extinction Coefficient
748600 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-NAMPT Aptamer (Biotin) (A320917) is a replica of the aptamer described in PMID: 22143382, N/A.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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