Anti-NADPH oxidase 4 Antibody (A81032)

Name: Anti-NADPH oxidase 4 Antibody
Brand: Antibodies.com
SKU: A81032
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to NADPH oxidase 4 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-NADPH oxidase 4 Antibody

Description

Rabbit polyclonal antibody to NADPH oxidase 4.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 479-578 of human NADPH oxidase 4 (NOX4) (NP_058627.2).

Sequence

LHNKFWQENRPDYVNIQLYLSQTDGIQKIIGEKYHALNSRLFIGRPRWKLLFDEIAKYNRGKTVGVFCCGPNSLSKTLHKLSNQNNSYGTRFEYNKESFS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, KOX-1, Renal NAD(P)H-oxidase, RENOX

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-NADPH oxidase 4 Antibody (A81032)

Western blot analysis of extracts of 293T cells, using Anti-NADPH oxidase 4 Antibody (A81032) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Western Blot - Anti-NADPH oxidase 4 Antibody (A81032)

Western blot analysis of extracts from normal (control) and NADPH oxidase 4 (NOX4) Rabbit polyclonal antibody knockout (KO) HeLa cells, using Anti-NADPH oxidase 4 Antibody (A81032) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunohistochemistry - Anti-NADPH oxidase 4 Antibody (A81032)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-NADPH oxidase 4 Antibody (A81032) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-NADPH oxidase 4 Antibody (A81032)

Immunohistochemistry analysis of paraffin-embedded mouse colon using Anti-NADPH oxidase 4 Antibody (A81032) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.