Figure 1: Western Blot - Anti-N-ethylmaleimide-sensitive fusion protein Antibody (A12714)
Western blot analysis of extracts of various cell lines, using Anti-N-ethylmaleimide-sensitive fusion protein Antibody (A12714) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 2: Immunofluorescence - Anti-N-ethylmaleimide-sensitive fusion protein Antibody (A12714)
Immunofluorescence analysis of BALB-3T3 cells using Anti-N-ethylmaleimide-sensitive fusion protein Antibody (A12714) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 3: Immunofluorescence - Anti-N-ethylmaleimide-sensitive fusion protein Antibody (A12714)
Immunofluorescence analysis of U-2 OS cells using Anti-N-ethylmaleimide-sensitive fusion protein Antibody (A12714) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 4: Western Blot - Anti-N-ethylmaleimide-sensitive fusion protein Antibody (A12714)
Immunoprecipitation analysis of 600µg extracts of mouse brain cells using 3µg of Anti-N-ethylmaleimide-sensitive fusion protein Antibody (A12714). This Western blot was performed on the immunoprecipitate using Anti-N-ethylmaleimide-sensitive fusion protein Antibody (A12714) at a dilution of 1:3000.
