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Anti-N Cadherin Aptamer [NC23] (Biotin) (A321194)

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Secondary Structure - Anti-N Cadherin Aptamer [NC23] (Biotin) (A321194) - Antibodies.com
$730
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Name
Anti-N Cadherin Aptamer [NC23] (Biotin)
Description
Synthetic DNA aptamer to N Cadherin [NC23] (Biotin).
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Phosphate Buffered Saline. Binding Temperature: 37°C.
Sequence
5'-dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdApdGpdApdCpdGpdApdGpdTpdTpdTpdTpdApdTpdTpdCpdApdTpdTpdTpdTpdCpdCpdApdTpdGpdTpdCpdGpdTpdApdGpdCpdTpdTpdApdCpdCpdGpdTpdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp-3'
Aptamer Length
76 nt
Aptamer Molecular Weight (Da)
23,361.2 Da
Aptamer Affinity
Affinity (Kd) = 174 nM
Extinction Coefficient
740200 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-N Cadherin Aptamer [NC23] (Biotin) (A321194) is a replica of the aptamer described in PMID: 33146506.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Secondary Structure - Anti-N Cadherin Aptamer [NC23] (Biotin) (A321194) - Antibodies.comBild Vergrößern
Figure 1: Anti-N Cadherin Aptamer [NC23] (Biotin) (A321194)
Secondary structure of Anti-N Cadherin Aptamer [NC23] (Biotin) (A321194).
PubMed LogoPublikation anzeigen
Ni-Nitrilotriacetic Acid Affinity SELEX Method for Selection of DNA Aptamers Specific to the N-Cadherin Protein
Verweise: Anti-N Cadherin Aptamer [NC23] (Biotin) (A321194)
Abstrakt:

Nucleic acid aptamers are single-stranded oligonucleotides that may be evolved for affinity and specificity for their targets and can be easily produced, regenerated, and stabilized. In this study, we adapted Ni-NTA (nickle-charged nitrilotriacetic acid) affinity-chromatography in the development of single-stranded DNA aptamers against N-cadherin protein by systematic evolution of ligands by exponential enrichment (SELEX). After ten rounds of selection, two aptamers, designated NS13 and NC23, were selected, which showed low dissociation constants of 93 and 174 nM, respectively. The 5'-carboxyfluorescein-labeled NS13 was used for the sensitive detection of N-cadherin protein by the enzyme-linked oligonucleotide assay (ELONA) method.

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