Recombinant mouse monoclonal [rMYOD1/6911] antibody to MyoD1.
Spezifität
MyoD1, one of the MyoD family of myogenic helix-loop-helix transcription factors, combined with myogenin, plays a role in coordinating the myogenic differentiation pathway from the determination of mesodermal precursors into myoblasts, the differentiation of myoblasts into myotubes, and finally the maturation of myotubes into skeletal myofibers. Normal mature skeletal muscle does not express MyoD1 protein. MyoD1 is expressed in myoblasts before differentiation while myogenin has post-differentiation functions. Anti-MyoD1 immunostaining identifies cells committed to myogenesis in their earliest phase, thus, it is a better biomarker for less differentiated Rhabdomyosarcomas (RMS). RMS are the most frequent malignant soft tissue neoplasms of childhood. While better differentiated RMS have cross-striations or rhabdomyoblasts that allow for a confident morphologic diagnosis, less differentiated RMS resemble other small blue round-cell tumors. Studies suggest, anti-MyoD1 may be used together with anti-Myogenin and anti-Desmin as a panel of markers since any RMS is virtually never negative for all three markers simultaneously.
Anwendungen
IHC-P
Verdünnungen
IHC-P: 1-2 µg/ml
Reaktivität
Human
Immunogen
Recombinant fragment, around amino acids 1-100, of human MyoD1 protein. The exact sequence is proprietary.
Wirt
Mouse
Klonalität
Monoclonal
Klon
rMYOD1/6911
Isotyp
IgG1
Leichte Ketten
kappa
Konjugat
Unconjugated
Reinigung
Protein A chromatography.
Konzentration
200 µg/ml
Molekulargewicht
45 kDa
Produktform
Liquid
Formulierung
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-MyoD1 Antibody [rMYOD1/6911] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
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