Anti-Myeloperoxidase Antikörper [D02-2A1] (A280193)

Anti-Myeloperoxidase Antibody [D02-2A1]

Mouse monoclonal [D02-2A1] antibody to Myeloperoxidase.

This antibody recognises myeloperoxidase (MPO), a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is largely expressed by neutrophils within azurophilic granules, but is also present in monocytes, macrophages and central nervous microglial cells. MPO is synthesized as a precursor protein that undergoes a series of modifications including cleavage and glycosylation to form the catalytically inactive MPO precursor. Subsequently an iron-heme is incorporated into the protein resulting in the catalytically active MPO. In response to bacterial infection, neutrophils containing MPO fuse with the phagosome and when the common membrane is ruptured, MPO is release into the phagosome. MPO then uses H2O2 to generate the bactericidal compound, hypochlorous acid (HClO). MPO deficiency is associated with a higher occurrence of severe infections and chronic inflammatory processes, although ~50% of individuals are asymptomatic (Antachopoulos 2010).The purified Mouse anti Human myeloperoxidase antibody, clone D02-2A1 (MCA6260GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human myeloperoxidase antibody, clone D08-8G5 (MCA6261B) as the detection antibody.

ELISA

ELISA: 1 µg/ml

Human

Purified native human myeloperoxidase.

Mouse

Monoclonal

D02-2A1

IgG1

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human myeloperoxidase antibody, clone D02-2A1, recognizes myeloperoxidase (MPO), a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is largely expressed by neutrophils within azurophilic granules, but is also present in monocytes, macrophages and central nervous microglial cells (Strzepa et al. 2017). MPO is synthesized as a precursor protein that undergoes a series of modifications including cleavage and glycosylation to form the catalytically inactive MPO precursor. Subsequently an iron-heme is incorporated into the protein resulting in the catalytically active MPO (Hansson et al. 2006). In response to bacterial infection, neutrophils containing MPO fuse with the phagosome and when the common membrane is ruptured, MPO is release into the phagosome. MPO then uses H2O2 to generate the bactericidal compound, hypochlorous acid (HClO) (Strzepa et al. 2017). MPO deficiency is associated with a higher occurrence of severe infections and chronic inflammatory processes, although ~50% of individuals are asymptomatic (Antachopoulos 2010).The purified Mouse anti Human myeloperoxidase antibody, clone D02-2A1 (MCA6260GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human myeloperoxidase antibody, clone D08-8G5 (MCA6261B) as the detection antibody.

84 kDa myeloperoxidase, 89 kDa myeloperoxidase, EC 1.11.1.7, EC1.11.2.2, fj80f04, MPO, mpx, myeloid-specific peroxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain, PERM_HUMAN, wu:fj80f04