Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
COI, COX I, COX1, COX1_HUMAN, COXI, Cytochrome c oxidase polypeptide I, Cytochrome c oxidase subunit 1, Cytochrome C Oxidase subunit I, Mitochondrially encoded cytochrome c oxidase I, MT CO1, MT-CO1, MTCO 1
Figure 1: Western Blot - Anti-MTCO1 Antibody (A12131)
Western blot analysis of NIH/3T3, using Anti-MTCO1 Antibody (A12131) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-MTCO1 Antibody (A12131) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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