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Maus SynGAP Kit Elisa (A327039)

$682
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Vorlaufzeit
Lieferung in 7-10 Werktagen
Telefon
+1 (314) 370-6046
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Email
orders@antibodies.com
Produktname
Mouse SynGAP ELISA Kit
Beschreibung
Mouse SynGAP ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of mouse SynGAP in serum, plasma, and other biological fluids.
Assay-Typ
Sandwich (quantitative)
Assay-Prinzip
Mouse SynGAP ELISA Kit (A327039) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse SynGAP in serum, plasma, and other biological fluids. An antibody specific for SynGAP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the SynGAP present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-SynGAP Antibody, which binds the captured SynGAP present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of SynGAP captured in each well. The concentration of SynGAP can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Plattform
Pre-coated Microplate (12 x 8 well strips)
Nachweismethode
Colormetric
Instrument
Colorimetric Microplate Reader
Probentyp
Serum, plasma, and other biological fluids.
Sensitivität
46.88 pg/ml
Detektionsbereich
78.13-5000 pg/ml
Testdauer
4h 30m
Reaktivität
Mouse
Kreuzreaktivität
No significant cross-reactivity or interference between mouse SynGAP and analogues was observed.
Recovery
Sample Type n Range Average
Serum 8 94% - 108% 101%
EDTA Plasma 8 93% - 110% 100%
Cell Culture Media 8 93% - 109% 100%
Linearity
Sample Type 1:2 1:4 1:8 1:16
Serum (n=5) 95-112% 92-103% 87-103% 90-102%
EDTA Plasma (n=5) 97-114% 81-91% 87-100% 82-96%
Cell culture media (n=5) 94-106% 84-96% 85-100% 84-95%
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Allgemeine Hinweise
Information online is representative. Always refer to the Product Manual inside the kit.
Lagerung
Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.
Synonyms
DKFZp761G1421, KIAA1938, MRD5, Neuronal RasGAP, OTTHUMP00000064825, p135 SynGAP, Ras GTPase activating protein SynGAP, Ras GTPase-activating protein SynGAP, RASA 1, RASA 5, RASA1, RASA5, SYGP1_HUMAN, Synaptic Ras GAP 1, Synaptic Ras GTPase activating protein, Synaptic Ras GTPase activating protein 1, Synaptic Ras GTPase activating protein 1 homolog, Synaptic Ras GTPase activating protein 135kDa, Synaptic Ras GTPase-activating protein 1, Synaptic Ras-GAP 1, SYNGAP 1, SYNGAP1
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
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