This IL10 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-IL10 Antibody. Standards or samples are then added to the microtire plate wells and IL10, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of IL10 present in the sample, Anti-IL10 Antibody (Biotin) is added to each well to "sandwich" the IL10 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL10 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Serum, body fluids, tissue lysates and cell culture supernatants.
Sensitivität
< 1 pg/ml
Detektionsbereich
15.6-1000 pg/ml
Reaktivität
Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at 2-8°C for 6 months or at -20°C for 12 months.
